Red blood cell (RBC) fractions were studied after separation of whole blood by means of counterflow centrifugation, Percoll column (Pharmacia, Uppsala, Sweden), and a combination of both separation techniques. Mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean ...
The cell membrane of red blood cells is composed of lipids and proteins that gives the cells stability and the ability to deform under stressful conditions without rupturing. In humans, the red blood cells are devoid of any organelles or nucleus and can be thought of as a sack of haemoglobin...
Spleen marginal zone (MZ) B cells are important for antibody responses against blood-borne antigens. The signals they use to detect exposure to blood are not well defined. Here, using intravital two-photon microscopy in mice, we observe transient contacts between MZ B cells and red blood cells...
was calculated according to the level of white blood cell and platelet depletion from whole blood samples. Dextran sedimentation of whole blood resulted in 90.4 ± 11.0% and 98.7 ± 2.0% depletion of white blood cells and platelets respectively from the enriched RBC fractions. ...
We first pooled the proteasome-containing fractions (fractions 10–21) for higher resolution analysis by cryoelectron microscopy (cryo-EM). By single-particle analysis, we obtained a reconstruction of the 20S proteasome directly from RBCs with a nominal resolution of 3.35 Å, confirming an intact...
The three resulting red blood cell fractions then may be added to a liquid medium, such as RPMI-1640, MEM, BME, Ham F12, MCDB104, MCDB153, and the like, and can be applied to various research projects and studies, such as observations of their effects and actions on various bacteria. ...
for 1 h, and the low molecular weight fractions collected in airtight syringes containing 1 μM glutathione in 0.5 N HCl. Under these conditions, any nitrite present was converted to S-nitrosoglutathione, which was then measured by photolysis-chemiluminescence (detection limit ˜1 nM). SNO-Hb ...
A system for collecting and processing donated blood comprises a first porous medium interposed between a blood collection bag and a satellite bag and a se... DB Pall,TC Gsell - US 被引量: 304发表: 1992年 加载更多研究点推荐 red blood fractions red blood cells 引用走势 2007 被引量:4 ...
The shift is in agreement with the finding of significantly higher values for the microcyte fraction, as well as with significantly lower mean cell volume (MCV) values. No significant changes were observed for the macrocytic fractions of red blood cells....
In contrast, the IC 50 for artemisinin was significantly higher in 'old' thalassaemic RBC but was similar in the 'young' and 'intermediate' fractions and whole blood. These findings suggest that oxidant stress plays a role in mediating the protection against malaria in thalassaemic RBC. Vitamin...