Fig. 1. Diagram of the red blood cell (RBC)-hitchhiking strategy. Various (different materials, sizes, and material coatings) ivermectin (IVM)-loaded nanoparticles were prepared and adsorbed onto the RBC surface in a non-covalent manner to form a RBC-nanoparticles complex. The RBC-hitchhiked ...
A suspension of 2 × 106 leukocyte/mL (DAUDI) labeled with hexidium iodide (520/600 nm, Molecular Probes, Eugene, OR) in 30% hematocrit RBCs was used in the experiments. The suspending medium was either saline or a dextran solution (3% by weight, 300 kDa) (Cokelet and Goldsmith, 1991...
The behaviors of MZ B cells were observed with intravital two-photon microscopy inCd19−/−mice reconstituted with Ub-GFP+B cells. CTV-labeled naive B cells were used to identify follicles. PKH26-labeled RBCs were intravenously injected 3 h before imaging, and 70-kD Texas Red dextran w...
The Ca ionophore A23187 (Calbiochem, La Jolla, CA, 2.4 mM stock solution in 100%ethanol) was added to a final concentration of 1 pM to 3 ml of 0.05% HCT suspensions of DPH-labeled cells in cell wash medium containing 0-5 mM C h . After 15 min, DPH fluores- cence anisotropy in ...
Fringes in red blood cell ghosts (a) and intact red blood cells (b, d, e) from multiplanar excitation of fluorescence at the standing-wave antinodes. The cells were stained with the membrane-specific dyes DiI (a and b), DiO (d) or DiI(5) (e), and mounted on top of a mirror ...
A solution container was only labeled 0.005 M. When phenolphthalein was added onto the solution, the color of the solution turned pink. After identifying the type of solution, it was later titrated with sodium carbonate. If the solution was sulphuric acid ...
peroxidase-conjugated reagents and fluorescent dye– or hapten-labeled tyramides to deposit a detectable product at the site of enzymatic activity. We have used our extremely versatile Amplex Red reagent—the most stable and sensitive fluorogenic substrate known fo...
Even, manual pressure was applied and the percentage of reticulocytes that traversed the system was evaluated using flow cytometry through the detection of CellTracker Green CMFDA-labeled cells. The results for this experiment are shown in Figure 2A. Figure 2. GSK3 and Lyn inhibition impacts ...
Fringes in red blood cell ghosts (a) and intact red blood cells (b, d, e) from multiplanar excitation of fluorescence at the standing-wave antinodes. The cells were stained with the membrane-specific dyes DiI (a and b), DiO (d) or DiI(5) (e), and mounted on top of a mirror ...
(a) Structural diagram of ICG and Hb complex. Hb consisted of four polypeptide subunits, and ICG molecule bound at the site close to subunit A (yellow) and subunit B (purple) of Hb. ICG was surrounded by 4 hemes, and the oxygens carried on hemes were easily transferred to boosted PDT...