53. This suggests that within the Rab4/Rab11 pathway multiple sub-pathways exist. Recently, a new protein complex, retriever, has been identified that associates with SNX17 on EEs and sorts α5β1 integrin from the early endosome to the recycling pathway54. Retriever is therefore a likely ...
Cell lysates were prepared using NativePAGE Sample Prep kit (ThermoFisher Scientific). In brief, the cells were lysed on ice for 10 mins in 1 × sample buffer containing 1% Digitonin and Halt protease and phosphatase inhibitor cocktail followed by spin at 20,000×gfor 30 mins. Protein...
As a recycling endosome protein that also interacts with the AMPAR complex, we hypothesize that GRASP1 may be a key molecule essential for experience-dependent remodeling of synaptic transmission and structure necessary for proper learning and memory. Here, we report that Grasp1 knockout (KO) mice...
Strikingly, the vRNPs co-localized with Rab11a and concomitantly moved with Rab11a or transferrin in infected cells, suggesting that SeV utilizes the recycling endosome pathway regulated by Rab11a to deliver vRNPs to the cell surface (Chambers and Takimoto, 2010). Similarly, recent studies on ...
EHD1 also maintains the perinuclear localization of glucose transporter 4 in cultured adipocytes [19] and positively regulates the kinetics of endosome-to-Golgi retrieval of the cation-independent mannose-6-phosphate receptor [20]. We previously suggested that EHD4 regulates the EE to ERC transport ...
its concentration in the endosome, and then its release (Fig. S5A). NP-encapsulated siLCN2 was transfected into OSCC cells for 48 h, and the protein was extracted to verify the successful downregulation of LCN2 expression (Fig.7F) and the inhibition of OSCC cell migration (Figs.7G and H...
We also show that depletion of SORL1 significantly impacts the endosomal recycling pathway for APP and GLUA1 at the level of the recycling endosome and trafficking to the cell surface. This has a functional effect on neuronal activity as shown by multi- electrode array (MEA). Conversely, ...
Early endosome antigen-1 EHD: Eps15 homology domain eHEV: Enveloped HEV EM: Electron microscopy ER: Endoplasmic reticulum ERC: Endocytic-recycling compartment ERGIC: Endoplasmic reticulum-Golgi intermediate compartment ESCRT: Endosomal sorting complexes required for transport ...
4G, H). In Atg5 knockout slices, rapamycin had no effect on LFS-induced GluA2 internalization and the recycling endosome decrease (Fig. 5e–g), indicating that LFS induces AMPA receptor internalization and reduces recycling endosomes in an autophagy-dependent manner. Moreover, CQ and leupeptin ...
Isolation of endosome-enriched mouse kidney membranes Mice were sacrificed by cervical dislocation. The removed kidneys were homogenized with 10 volumes homogenization buffer HB (10 mM Tris pH 7.6, 250 mM Sucrose, 5 mM EDTA, Complete) in a glas potter by applying∼15 strokes. The crude...