Human PPFIA1 cDNA (transcript variant 1, NM_003626) from Origene Technologies Rockville, MD, USA was used as a template. PPFIA1r mutant construct was obtained by PCR, according to the Taq polymerase manufacturer's instructions, using Phusion Site-Directed Mutagenesis Kit (Life technologies). ...
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Dudley Strickland (American Red Cross, Rockville, MD). PDGF-BB, EGF, basic fibroblast growth factor, and transforming growth factor-β1 were from R&D Systems. Interferon-γ was from Genzyme Diagnostics. Aprotinin and phenylmethylsulfonyl fluoride were from Sigma. Leupeptin and L-trans-epoxysuccinyl...