Primary antibodies included mouse anti-IBA1 (1:250, generated at the Cleveland Clinic Hybridoma Core, provided by Bruce Trapp), rabbit anti-ionized calcium binding adaptor molecule 1 (IBA1) (1:250, 019-19741; Wako, Richmond, VA), rat anti-mouse CD3 (1:100, eBioscience, 16-0032-85,),...
Transversal sections (10 μm) of soleus muscles were cut (CM1850 cryostat, Leica Microsystems, Nussloch, Germany) at −20°C and adhered to Snowcoat X-tra slides (Surgipath, Richmond, IL). After 1 hour in a blocking buffer, the sections were incubated for 2 hours with rat anti-mouse ...
overnight (1:10,000, 019–19741, Wako Labs, Richmond, VA), followed by Alexa Flour goat anti-rabbit 488 (1:1000, Invitrogen, Waltham, MA). To measure levels of phosphorylated tau, AT8 antibody was applied overnight (1:500, MN1020, Pierce Antibodies, Waltham, MA), followed by Alexa F...
Buttafinguz Member Aug 29, 2010 22 2 Richmond Feb 7, 2016 #20 A2CKilla said: A simple unplug and replug in fixes the issue...when you get to the adb point on the phone and you make sure the driver is installed on the computer correctly, simply unplug from the computer, replug...
(1:100; R184), mouse α laminin (1:100; 2E8; Developmental Studies Hybridoma Bank, USA [93]), mouse αβ-tubulin (1:300; Millipore Sigma, USA), mouse α CD45 (1:50; Agilent DAKO, Santa Clara, CA, USA), and rabbit α Iba-1 (1:800; Wako FUJI Film, Richmond, VA, USA),...