DNA Technology Vocab 老師11個詞語 mmshaw02wheeler 預覽 Writing Crosswords 1.5-1.8 44個詞語 Emmalousmith 預覽 BIOS 220 EXAM 2 VOCAB CH. 10 53個詞語 zeynepsuej 預覽 Chapter 9: Gene transfer and mutations 20個詞語 akesslerr 預覽 D
Recombinant DNA technology Allison, Ch8: Recombinant DNA technology and molecular cloning Historical perspective Labmda phage Restriction modification system Restriction enzymes DNA ligase Sticky end ligation Blunt end ligation Modified blunt end ligation Modified blunt end ligation Molecular cloning Vectors Car...
E.coli and T4 DNA encode the DNA ligase Seals the single stranded nicks between adjacent nucleotides in a duplex DNA chain The reaction catalysed by these ligases and similar in the host cell but differ in their cofactor requirement T4 needs ATP and E.coli NAD+ Both the enzyme catalyze the ...
. Complementary DNA was synthesized from 2 μg of total RNA using RevertAidTm First Strand cDNA Synthesis Kit (Fermentas) following the manufacturer’s protocol. RT-qPCR was performed in 20 μL reactions containing 10 μl SYBR Green Master Mix (Roche Applied Science, Germany), 0.6 μL (10 ...
REFERENCES Cohen et al. “Construction of biologically functional bacterial plasmids in vitro”. Hershfield et al. “Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA”. Wilson et al. “Molecular cloning of fragments of bacteriophage T4 DNA”. Aslanidis et al. “Ligase-in...