基因工程 第十六章 重组DNA技术 RECOMBINANT DNA TECHNOLOGY.ppt 18页VIP内容提供方:flyben 大小:630.5 KB 字数:约2.6千字 发布时间:2018-06-20发布于广东 浏览人气:17 下载次数:仅上传者可见 收藏次数:0 需要金币:*** 金币 (10金币=人民币1元)基因工程 第十六章 重组DNA技术 R
Type II (blunt ends) Blunt ends are 4-6bp long and pallidromic Means the base sequence in the second half of a DNA strand is the mirror image of the sequence in the first half 5’-GAA AAG-3’ 3’-CTT TTC S1 nuclease S1 nuclease cuts the single stranded DNA or single strand protru...
Restriction enzymes DNA ligase Sticky end ligation Blunt end ligation Modified blunt end ligation Modified blunt end ligation Molecular cloning Vectors Carry DNA molecules Independent replication (origin) Unique restriction sites (MCS) Selectable marker Easy to transfer and recover from host Choice of vect...
Recombinant DNA Technology MA Duan,M.D,Ph.D,Professor Recombinant DNA is a form of synthetic DNA that is engineered thro
Molecular Biology and Recombinant DNA Technology 热度: RecombinantDNAI Basicsofmolecularcloning Polymerasechainreaction cDNAclonesandscreening RecombinantDNATechnology •Utilizesmicrobiologicalselectionandscreening procedurestoisolateagenethatrepresentsaslittleas ...
Recombinant DNA cloning technologyChapter 7 阅读了该文档的用户还阅读了这些文档 58 p. 基于CAD的Geant4几何自动建模方法研究与实现 6 p. 小区监控设备的维护与保养方法 4 p. Donnan平衡和渗透压 5 p. 教案:4.3《宇宙的未来》(新人教版必修5) 4 p. 第七届全国信息技术应用水平大赛活动方案_16777 ...
Block II Lecture 1: Recombinant DNA Technology Part I. DNA Manipulations: Basic Techniques Overview of the Procedure Cloning Vectors Target GenCloning a
Mycobacterial DNA-binding protein 1 (MDP1, namely Rv2986c, hupB or HU) is a major Mycobacterium tuberculosis protein that induces vaccine-efficacy by co-administration with CpG DNA. To produce MDP1 for booster-vaccine use, we have created recombinant MDP1 produced in both Escherichia coli (...
This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker′s yeast. 货号:91077C-100MG 品牌:Sigma 是否进口:是 SCF Protein, Mouse, Recombinant (ECD, His Tag) 价格: ￥390/瓶 产品详情: SCF Protein, Mouse, Recombinant (ECD, His Tag)...
Controlling Gene Expression. Control Mechanisms Determine when to make more proteins and when to stop making more Cell has mechanisms to control transcription. GROUP 2 DNA TO PROTEIN. 9.1 RICIN AND YOUR RIBOSOMES. In Vitro Translation: The Basics ...