The proliferation of RCEC was detected by MTT assay and flow cytometry. The cyclin D1 mRNA was detected by reverse transcription polymerase chain reaction. RESULTS rhEGF stimulated RCEC proliferation in a time- and concentration-dependent manner and increased the S-phase cells fraction. rhEGF 0.1,...
Furthermore, the toxicity against red blood cells increased in a dose-dependent manner to approximately 25% when the peptide concentrations were doubled. The toxicity determination of javanicin is shown in Fig. 5. Figure 5 The red blood cell hemolytic assay for in vitro toxicity determination ...
Cyclin D1 Induction through IκB Kinase β/Nuclear Factor-κB Pathway Is Responsible for Arsenite-Induced Increased Cell Cycle G1-S Phase Transition in Hum... Environmental and occupational exposure to arsenite is associated with an increased risk of , including skin, urinary bladder, and respirato...
Depletion of GAS by RNA interference in MCF7 cells led to a decrease in the mRNA and protein levels of ER target genes such as pS2, c-Myc and cyclin D1, indicating the role of GAS in the regulation of ER target genes. GAS has also been found to associate with an SET1-...
Assay-dependent - 产品规格 种属反应 Human 宿主/亚型 Rabbit / IgG Expression System Expi293 分类 Recombinant Monoclonal 类型 Antibody 克隆号 10H10L15 抗原 full-length protein corresponding to human SMAD4 (aa 1-552) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0....
PCR analysis has showed downregulation of cyclin D1 and K-Ras. Subsequent treatment with docetaxel has exerted a synergistic antimitotic effect exhibited by BrdU and Ki-67. Flow-cytometry has reported diploid DNA. A biochemical assay showed activation of caspase-3/CPP32 pathway which led to ...
Additionally, our results from the antimicrobial assay, the microscopic analysis and the Pv D1r-FITC labeling assays corroborate each other and lead us to suggest that the target of Pv D1 in C. albicans cells is the sphingolipid glucosylceramide....
PCR analysis has showed downregulation of cyclin D1 and K-Ras. Subsequent treatment with docetaxel has exerted a synergistic antimitotic effect exhibited by BrdU and Ki-67. Flow-cytometry has reported diploid DNA. A biochemical assay showed activation of caspase-3/CPP32 pathway which led to ...
1A]. The levels of CDK4 and cyclin D1 that drive cells over the G1/S checkpoint were also detected to be upregulated by approximately 3- and 3.5-fold, respectively 6 h after the treatment [Fig. 1C,D]. The level of phosphorylated ATF-2 was found to be tripled at 60 min. ...
In other specific embodiments, in the presence of a constant suboptimal amount (6 nM) of soluble human CD40L, a CD40 agonist antibody has an EC50 (as measured in the B cell proliferation assay described in the examples) that is equal or less than the EC50 of mAb 1 (11B6) antibody. The...