Recombinant Anti-GPA33/A33 Antibody, Rabbit Monoclonal Validated Applications ApplicationDilution ELISA1:5000-1:10000 IHC-P1:100-1:500 Please Note: Optimal concentrations/dilutions should be determined by the en
In addition to hybridoma conversions, recombinant antibodies can be produced by directly cloning antibody sequences from B-cells. This development process involves: (1) immunization of host animal (rabbit or goat) with desired antigen, (2) isolation of B cells from the host animal, (3) ...
Using an anti-His-tag monoclonal antibody, Western blot analysis was performed to confirm the rhFNP expression and purification (Figure S1 D and E). The purity of the rhFNP protein was >90 % (Figure S1 F). 3.2. The rhFNP promoted cell adhesion and migration of HaCaT cells To ...
Blots were probed with 1/5000 dilution of rabbit anti-human HSPD1 antibody (Abcam, ab46798) in Tris-buffered saline containing 0.1% Tween 20 (TBST) and 1/5000 dilution in TBST of mouse anti-rabbit GAPDH monoclonal antibody (Abcam ab8245) (internal loading control)....
However, GAPDH was regulated in virus infected CEF cells at certain times, indicating that it could not be chosen for endogenous control. At 12 hpi, the amount of HSP70 in the rNA-wt infected cells was greatly increased relative to rSD20 infected cells and control cells. At 24 and 36 hpi...
PPAR gamma Polyclonal Antibody Affinity Cat#AF6284; RRID: AB_2835135 Phospho Erk1/2 (Thr202/Tyr204) Rabbit mAb Cell Signaling Technology Cat#4370; RRID: AB_10898026 Erk1/2 Rabbit mAb Cell Signaling Technology Cat#4695; RRID: AB_390779 Mouse monoclonal anti-CD36 Santa Cruz Biotechnology Cat...