A method for producing recombinant adeno-associated virus (AAV) in a cell comprises the step of culturing a cell containing; (a) a hybrid virus comprising: (i) adenovirus sequences comprising the adenovirus 5' and 3' cis-elements necessary for replication and virion encapsidation; (ii) AAV ...
Recombinant adeno-associated viruses (rAAV) are highly efficient vectors for gene delivery into the central nervous system (CNS). However, host inflammator... Mihail,Y.,Mastakov,... - 《Journal of Virology》 被引量: 170发表: 2002年 Recombinant adeno-associated virus-mediated global anterograde...
In this article, we describe a set of recombinant adeno-associated viruses (AAVs) that can deliver cDNAs encoding the GABA-producing enzyme, glutamate decarboxylase (GAD), directly into neural cells. We have characterized these recombinant AAVs in several cell lines derived from the CNS. These ...
Recombinant Adeno-Associated Virus-mediated rescue of function in a mouse model of Dopamine Transporter Deficiency Syndrome P. Illiano, C.E. Bass, L. Fichera, L. Mus, E.A. Budygin, T.D. Sotnikova, D. Leo, S. Espinoza & R.R. Gainetdinov Scientific Reports volume 7, Art...
Considering the low immunogenicity, high gene delivery efficacy, and replication deficiency characteristics11, it may be ideal to use recombinant adeno-associated viruses (rAAVs) to deliver and express GSDMNT gene in the tumor cells. rAAV has been widely used in clinical gene therapy for many ...
The adeno-associated virus is a dependoparvovirus and requires several host factors and the essential genes provided by a helper virus to replicate.38Several helper viruses in addition to the adenovirus were identified and characterized to support propagation, such as ...
Recombinant adeno-associated virus as vaccine delivery vehicles Adeno-associated viruses (AAV) are non-enveloped, replication defective, single-stranded DNA virus and require co-infection with a helper virus, such as ad... K Vig,R Herzog,D Martin,... - 《Gene Therapy & Molecular Biology》 被...
Cell-Type-Specific Characteristics Modulate the Transduction Efficiency of Adeno-Associated Virus Type 2 and Restrain Infection of Endothelial Cells Adeno-associated viruses (AAVs) are promising vectors for various gene therapy applications due to their long-lasting transgene expression and wide spectru.....
efficient recombinant adeno-associated virus production by a stable rep-cap hela cell line correlates with adenovirus-induced amplification of the integrated rep-cap genome 热度: Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses ...
8.Culture of low passage 293 cells for the package of replication-deficient ade noviruses用于包装复制缺陷性腺病毒的低代次293细胞的培养 9.Construction of Human Adeno-associated Virus Type2 (AAV-2) Based Vectors, Expression of Foreign Genes in the Vectors and a Study on the Recombinant AAV Pack...