(Rec Room Image) The makers of the popular social app Rec Room filed a lawsuit in Seattle this week against a teenage user of the service, alleging that
The basic plot is notably slim, born from a 35-page screenplay with plenty of room for improvisation, and essentially hinges on a trio of plucky college kids running off into the woods to look for a local legend and then finding, well, what exactly when they get there? By playing on it...
To prepare 1% w/v and 2% w/v chitosan solutions, 1 g of chitosan was stirred in distilled water containing 1% v/v acetic acid for 1 h at room temperature. Simultaneously, 0.375 g of starch was stirred in distilled water at a temperature at 90 °C for 1 h to obtain a 1.5% w/v ...
and The Game Bakers’Furiis full of them. Part bullet-hell and part intense hack-and-slash action game,Furiis one of the best boss rush games out there. It’s full of style, it’s surprisingly challenging, and it’s very rewarding to master. Typically priced at $20,Furiis discountedby...
Blocking – 1 h at room temperature in 10% horse serum (Thermo Fisher, 16210064), 1% w/v bovine serum albumin (BSA) (Sigma, A2153) in PBS 1X. Primary antibody incubation was carried out overnight at 4 °C first using a 1:150 dilution of rabbit TSPO antibody (biorbyt, orb5845...
she’d be traveling with a circus as a lemur trainer or even have opened up a gallery to share her artwork with the world. Linus believed the world was better off with the paintings staying in this room, but he kept the thought to himself. He wasn’t there to engage in amateur art ...
Abstract Organic alcohols as very volatile compounds play a crucial role in the air quality of the atmosphere. So, the removal processes of such compounds are an important atmospheric challenge. The main goal of this research is to discover the atmospheric relevance of degradation paths of linear ...
(PBS, Corning, 46-013-CM) buffer for 10 min, then permeabilized with 0.1% Triton-X (Sigma-Aldrich, T8787), and blocked with BSA 5 mg/mL (VWR, VWRV033) for 10 min, all at room temperature. The cells were pre-equilibrated with hybridization buffer (10% formamide, 2×SSC (...
Cells were grown on coverslips and mock-treated or irradiated with 10 J m−2UV. RNA was labeled at the indicated time points for 1 h with 200 μM EU, fixed with 3.7% formaldehyde (FA) in PBS for 15 min at room temperature and permeabilized by 0.1% Triton X-100 in PBS...