are detected, allowing us to reach out directly with a reminder of maximum occupancy and quiet hours. This technology is privacy compliant, and only monitors the presence of decibels and devices-not any personal conversation or information. Thank you for supporting our efforts to be good neighbors...
Two-plex fluorescence in situ hybridization Thermo Fisher Scientific provided a ViewRNA ISH Tissue Assay kit (two plex) for use on mouse PDAC tumor tissue samples. Briefly, samples were first permeabilized with controlled protease digestion, followed by incubation with proprietary probe-containing soluti...
Samples were barcoded using the Cell-IDTM 20 Plex-Pd Barcoding Kit (Fluidigm), following the manufacturer’s instructions. The 20 barcoded samples were then pooled, resulting in a total of seven barcodepools. To minimize batch effects, all sequential samples collected from the same patient ...
, and then labeled with the iTRAQ Reagents 4-plex Kit (Applied Biosystems) according to the manufacturer’s protocol. Two independent biological replicates were applied for the iTRAQ experiment. The iTRAQ-labeled samples were combined, lyophilized, and fractionated with high-pH reversed-phase ...
Cytokine analysis in cell culture supernatants was performed using a magnetic bead-based 48-plex Luminex kit from MilliporeSigma (MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A, Cat# HCYTA-60K-PX48) or 4-plex Luminex kit for CXCL10, IFNβ, IL-6, TNFα from R&D Systems ...