Full size image As usual in biological electron bifurcations51,52,53,54, this endergonic charge transfer step could be driven by the initial exergonic PCET step (Fig.5d). The energetics suggests that the second PCET step is rate-limited by the proton transfer followed by a rapid non-adiaba...
Full size image The tendency to burst appears to be sensitive to the architecture of the proteins. No bursting events were observed for CRY-ADF3-CRY (Supplementary Movie 1). For CBM-ADF3-CBM (Supplementary Movie 2) all condensates that were aspirated did burst, whereas for FN-ADF3-FN (Su...
To better understand the mechanisms leading to increased mortality, we filmed the sexual interactions of the mosquitoes at a high resolution (1080 P). Females were harassed when sex ratios were biased towards males (see Supplementary Movie1). At the highest male-to-female ratio of 99:1, fem...
Super-resolution movies were Fourier-binned by 2 during movie alignment. Auto-picked particles were cleaned up through two rounds of 2D classification, and particles in the selected 2D classes were subject to ab initio reconstruction with C1 symmetry. Up to 4 ab initio maps were generated to ...
For the initial automated detection, movies were divided into segments of 100 frames each, the average intensity projection of each segment was computed and the resulting resampled movie was used for detection. For the detection of pyramidal cells, the PlaneCA1PC method of sima was run on the ...
the structures reported by these studies are very similar to ours using the full-length human Bre1 complex without any protein engineering; one RING subunit binds stably to the acidic patch via arginine anchors, and the other binds flexibly to the nucleosomal DNA. The three studies complement wit...
Full size image We next examined the orientation of the 23S L1 stalk in theE. faecalis70S structures. The L1 stalk is a dynamic structure of the 23S rRNA that interacts with the E site tRNA. An extensive analysis of stalk rRNA conformations in ribosome structures in the PDB classified the ...
Non-coding transcription at the intergenic regulatory regions is a prevalent feature of metazoan genomes, but its biological function remains uncertain. Here, we devise a live-imaging system that permits simultaneous visualization of gene activity along
. In contrast, almost all ofmSerpinb3a−/−FIECs died with rapid disappearance of endolysosomal staining prior to SG uptake (Fig.1h–kand Supplementary Movie4). Fig. 1: MouseSerpinb3aprotected fetal intestinal epithelial cells (FIECs) from lysoptosis-like death....
1e; Supplementary Movies 4 and 5). Rapid recovery of fluorescence after full bleach of ERC1 condensates (t1/2 = 5.6 ± 0.56 s; 68% mobile fraction) revealed fast exchange of ERC1 between condensates and cytoplasm (Fig. 1f,g; Supplementary Movie 6). Interestingly, the N-...