To better understand the mechanisms leading to increased mortality, we filmed the sexual interactions of the mosquitoes at a high resolution (1080 P). Females were harassed when sex ratios were biased towards males (see Supplementary Movie1). At the highest male-to-female ratio of 99:1, fem...
Figure 5a and Supplementary Movie 5 show the model structure of the RINGA-RINGB-Rad6A ubiquitin bound to the nucleosome based on Model I, where RINGA binds the acidic patch. In this model, the lysine residue closest to ubiquitin G76 is H2BK120 (Fig. 5b, Supplementary Movie 5), and the...
We then used relion_project to generate maps of these sub-sets and used Relion’s Refine 3D to generate high resolution maps. The maps for the three bodies were combined into a single map using UCSF Chimeravop maximumfunction. Local resolution was estimated using the MonoRes program as impleme...
For the initial automated detection, movies were divided into segments of 100 frames each, the average intensity projection of each segment was computed and the resulting resampled movie was used for detection. For the detection of pyramidal cells, the PlaneCA1PC method of sima was run on the ...