实时荧光定量PCR技术(Real-time quantitative Polymerase Chain Reaction简称Real Time PCR)是在定性PCR技术基础上发展起来的核酸定量技术。主要是在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,使每一个循环变得“可见”,最后通过Ct值和标准曲线对样品中的DNA(orcDNA)的起始浓度进行定量的方法。实时荧...
MY-B020G-4 96 wells Real-time quantitative PCR detection system,real time pcr machine price Max heating rate 6.1°C/sec Sample capacity(wells) 96 Reaction Volume 0-100 μL Consumables 0.2ml 96-well Plates(unskirt); 0.2ml 8-strip tubes ,0.2ml ...
2012. Quantitative real-time PCR (qPCR) detection chemistries affect enumeration of the Dehalococcoides 16S rRNA gene in groundwater. J. Microbiol. Methods 88:263-270. http://dx.doi.org/10 .1016/j.mimet.2011.12.005.Hatt, J.K. and Loffler, F.E. (2012). Quantitative real-time PCR (...
Real-time quantitative pcr detection tyr into the trp-1, mysion va and rab27a expression at the gene level. 翻译结果2复制译文编辑译文朗读译文返回顶部 real-time quantitative PCR TYR detected, TRP Mysion Va - 1, 27 and a Rab , in gene expression levels. ; ...
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1、实时荧光定量PCR(Quantitative Real-time PCR, qPCR) Real-time-PCR 和 qPCR 是一码事。实时荧光定量PCR,也叫Real-Time PCR,即二代PCR,是指在PCR扩增反应体系中加入荧光染料或者荧光基团,在整个PCR过程中通过收集荧光信号实时监测每一个循环中扩增产物量的变化,最后通过标准曲线和CT值对待测样品进行定量分析。
荧光定量PCR是指在PCR反应体系中加入荧光物质,利用荧光信号积累实时监测整个PCR进程,对未知模板进行定量分析的方法。由于在PCR扩增的指数时期,模板的Ct值和该模板的起始拷贝数存在线性关系,所以成为定量的依据。具有应用范围广、灵敏度高、操作简便、特异性和可靠性更强、自动化程度高、无污染性、具实时性和准确性等特...
Real time PCR的应用 mRNA表达的研究 微小残留病变的检测 肿瘤耐药基因表达的研究 病毒感染的定量监测 Vs普通PCR,RT-PCR的优势 采用封闭的检测模式,因此扩增产物导致污染的可能性比普通PCR要小得多 。 扩增产物的检测在PCR扩增过程中同时进 Real time PCR的应用 ...
实时荧光定量PCR 1. Detection of abundance of Pseudomonas in environmental samples by real-time quantitative PCR; 实时荧光定量PCR法检测环境假单胞菌属细菌丰度 2. Application of gene microarray and real-time quantitative PCR in detecting human papilloma virus; 基因微阵列分型与实时荧光定量PCR检测人乳...