The invention discloses a real-time fluorescence PCR (polymerase chain reaction) detection method based on ITS (internal transcribed spacer) gene for Opogona sacchari, comprising the following steps of: extracting the DNA of a sample to be detected, preparing the DNA into a template, performing ...
用RT-PCR简写表示qPCR可以引起混乱,并且与传统RT-PCR的平常应用不符。 1.2内参基因 内参基因应当指的是参照基因(reference genes),而不是管家基因(housekeeping genes)。 1.3 TaqMan探针 TaqMan探针应指的是水解探针(hydrolysis probes)。 1.4 FRET probe FRET probe (fluorescence resonance energy transfer probe,荧光...
实时荧光定量PCR原理与分析方法Real-time,fluorescence 匠心品质，快乐科研 Good Science ，G ood Products 实时荧光定量PCR原理与分析方法Real-time, fluorescence-based quantitative PCR 孟文举 上海翊圣生物科技有限公司产品主管 2018.09
Aiming at ribosome 18S rRNA gene sequence of apricot, the method designs the primer and probe for detecting apricot gene; and components of apricot in food and beverage are quantitatively detected by a real time PCR (Polymerase Chain Reaction) method. The real-time fluorescence PCR quantitative ...
Real-time fluorescent PCR 翻译结果5复制译文编辑译文朗读译文返回顶部 Real-time fluorescence PCR 相关内容 a各种形状和大小使家庭娱乐眼花缭乱 各種類の形およびサイズは家族の催し物を眩ます[translate] aturn key project 转动关键工程[translate]
实时荧光PCR基本原理 目前的实时荧光PCR技术主要是基于荧光共振能量转移(fluorescence resonanceenergy transfer,FRET)的原理,一对合适的荧光物质可以构成一个能量供体 (donor) 和能量受体 (acceptor) 对, 其中供体的发射光谱与受体的吸收光谱重叠,当它们在空间上相互接近到一定距离(1~10 nm)时,激发供体而产生的荧光能量...
CAS号: 英文名: Real-time fluorescence quantitative PCR (REALTIME PCR) 英文别名: Real-time fluorescence quantitative PCR (REALTIME PCR) 中文名: 实时荧光定量PCR(REALTIME PCR) 中文别名: 实时荧光定量PCR(REALTIME PCR) CBNumber: CB25819380 分子式: 分子量: 0 MOL File: Mol file ...
实时荧光PCR 1. In this paper,based on the cloning of ACO gene in Erianthus arundinaceus,the expression of ACO gene in Erianthus arundinaceus under ABA and ethephon treatments was investigated byReal-time fluorescence PCR. 在项目组克隆斑茅ACO基因的基础上,应用实时荧光PCR技术分析斑茅ACO基因在A-...
方法用实时荧光定量RT-PCR方法分析正常肝细胞系、肝癌癌旁细胞系及肝癌癌细胞系中DNMT1、DNMT3A、DNMT3B的表达谱,以及DNMT3B表达抑制后在肝癌细胞系SMMC-7721中出现表达上调的与肿瘤发生相关的基因PDCD4和MBD3的表达。 更多例句>> 5) Real-time fluorescence quantitative RT-PCR 实时荧光定量RT-PCR 1. Quantifica...
Fluorescence real-time quantitative gene amplification instrument PCR Description This product has many advantages such as a scientific and efficient temperature control system and photoelectric system, powerful and easy-to-use software analysis functions, and humanized control methods. It ...