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real-time fluorescence quantitative PCR for Staphylococcus aureus was established routinely.Results It was identified that femB gene fragment was cloned as standard successfully.Real-time fluorescence quantitative PCR method showed good specificity,sensitivity,stability by the verification experiment.And the ...
Objective To explore the rapid molecular diagnosis of enterovirus 71(EV71) infection.Methods A total of 871 cases of hand-foot-and-mouth disease were detected EV71 infection in vesicle fluid and throat swabs with real-time fluorescence quantitative PCR(RT-PCR).The patients with RT-PCR comfirmed ...
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Real-time fluorescent quantitative PCR, in PCR reaction system by adding fluorescent dyes or fluorescent probes, real-time monitoring of fluorescence signal accumulation process of the PCR reaction, standard curve by quantitative analysis of the unknown template, simple, fast, efficient, high-throughput...
Real-time fluorescence and deformability cytometry P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner, O Otto, C Herold, ... Nature Methods 15 (5), 355-358 312018 Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging ...
Moreover, a comparison of 2AP fluorescence in repeat versus mixed sequences indicates that bases in a RecA repetitive DNA filament exhibit higher degrees of freedom that might mediate a 'non-planar hydrogen bonding cross talk' across the bases on either strand. We discuss a model to explain ...
Intraoperative fluorescence enables highly specific real-time detection of tumours at the time of surgery. In particular, near-infrared (NIR) fluorescence is a promising tool currently being tested in clinical settings. Zhang et al. discuss the latest de
The platform takes advantage of synergistically enhanced colorimetric loop-mediated isothermal amplification (LAMP) assay and smartphone-based color analysis, enabling simple, rapid and reliable nucleic acid quantification without need for expensive fluorescence detection equipment. The SCPT platform has been ...
Fig. 1: Strategy, structures, and fluorescence properties of the PSMA-activatable probes. Results Design and synthesis of PSMA-targeted activatable probes FMRs based on benzonitrile and julolidine moieties are well known24. Recently, these have been successfully modified by Yen-Pang Hsu et al. and...