Protocol: Real-Time (Quantitative) PCR Amplification of cDNAe.g
Real-time PCR combines PCR amplification and detection into a single step. This eliminates the need to detect products using gel electrophoresis, and more importantly it enables the method to be truly quantitative. With real-time PCR, fluorescent dyes are used to label PCR products d...
We describe a simple, quantitative assay for any amplifiable DNA sequence that uses a video camera to monitor multiple polymerase chain reactions (PCRs) simultaneously over the course of thermocycling. The video camera detects the accumulation of double-stranded DNA (dsDNA) in each PCR using the ...
for accurate DNA quantification. Real-time quantitative PCR can be used with different quantitative tools such as DNAbinding dyes (Morrison et al. 1998), fluorescent oligonucleotides (Whitcombe et al. 1999), molecu 目标脱氧核糖核酸的PCR放大作用和实时PCR产品量化是准确脱氧核糖核酸量化的二个最常用技术...
Development of a multiplex real-time PCR assay with an internal amplification control for the detection of Campylobacter spp. and Salmonella spp. in chicken... J Alves,EY Hirooka,TCRMD Oliveira - LWT - Food Science and Technology 被引量: 12发表: 2016年 Development of a multiplex real-time ...
Real-time PCR assays used for quantitative RT-PCR combine the best attributes of both relative and competitive (end-point) RT-PCR in that they are accurate, precise, capable of high throughput, and relatively easy to perform.
实时荧光定量PCR原理与分析方法Real-time,fluorescence 匠心品质，快乐科研 Good Science ，G ood Products 实时荧光定量PCR原理与分析方法Real-time, fluorescence-based quantitative PCR 孟文举 上海翊圣生物科技有限公司产品主管 2018.09
The real-time assay will be useful for monitoring HBV-infected patients in routine diagnostic laboratories and in clinical practice enabling analysis of a wide dynamic range of HBV DNA in a single, undiluted sample. 展开 关键词: HBV DNA quantitation Real-time PCR Internal control ...
Our assay has important clinical applications, providing accurate diagnostic results in less time, from less biopsy material and at less cost than assays currently employed such as FISH or Southern blotting. 展开 关键词: Real-time PCR SYBR-green rearrangement amplification deletion ...
To test this system, we simulated transgene flow using known quantities of GE and non-GE DNA and determined that 0.038% (38 pg) GE papaya DNA could be detected using real-time PCR. We also validated this system by extracting DNA from known ratios of GE seeds to non-GE seeds of papaya...