According to the real-time fluorescence quantification PCR method, a specificity quantification PCR detection primer and a Taqman probe are designed according to a bE gene of the ustilago scitaminea; with the adoption of the real-time fluorescence quantification PCR method, the quantity of the ...
PCR 的 100 倍(图 3 )。 3 份不同稀释度模板组内变 异系 数为 1.061%～2.873% ,组间变异系数为 0.397%～3.829% (表 1 ),重复性良好。 · 601 · 贵州农业科学 GuizhouAgriculturalSciences 表 1 实时荧光定量 PCR 的 Ct 值 Table1TheCtvalueofSYBRGreenⅠreal timefluorescencequantificationPCR 组名 Gr...
* 4-channel fluorescence detection, no cross interference between channels. * User-friendly and fully functional software, flexible program setting, comprehensive analysis and reporting functions, all the parameters can be stored. Real-time PCR hardware:Thermoelectr...
建立的洋虫β-actin基因荧光定量RT-PCR方法简便、特异性强,该体系的建立可用于洋虫蜕皮相关基因 表达差异的深入研究。 关键词:洋虫;β-actin基因;荧光定量RT-PCR;SYBRGreenI 中图分类号:Q78文献标志码:A论文编号:2012-2695 EstablishmentofReal-timeFluorescenceQuantitativeRT-PCRforβ-actinGene ofPalembusdermestoid...
vivo, thus delaying the treatment. In response to novel coronavirus infection caused by severe acute respiratory infection, the WHO gave a real-time fluorescence quantitative PCR detection program in January 17th. Nucleic acid quantification based on PCR is one of the fastest methods for virus ...
乙型肝炎病毒核酸的实时荧光定量PCR检测及其临床意义 目的建立实时荧光定量PCR(real-time fluorescence quantitative PCR,RFQ-PCR)检测HBV-DNA含量的方法,探讨其在乙型肝炎预防,诊断,治疗及判断病情等方面的临床应用价值... 王旭东,张冬雷,李立人,... - 《现代检验医学杂志》 被引量: 0发表: 2006年 加载更多研究...
Objective To establish real-time fluorescence quantitative reverse transcription-polymerase chain reaction(RFQ-RT-PCR) for HCV RNA by using double-probe,and to resolve the problems of sensitivity and specificity of HCV RNA quantification.Method A total of 89 anti-HCV positive cases and 220 anti-HCV...
1.1 实时定量PCR的简写 建议实时定量PCR (quantitative real-time PCR) 应当简写为qPCR,反转录PCR (reverse transcription–qPCR) 应当简写为RT-qPCR。用RT-PCR简写表示qPCR可以引起混乱,并且与传统RT-PCR的平常应用不符。 1.2 内参基因 内参基因应当指的是参照基因(reference genes),而不是管家基因(housekeeping genes...
实时荧光定量PCR仪qTOWER（RealtimePCR） Quantitative real-time rapidPCR qTOWER | Quantitative real-time rapidPCR Combination of rapidPCR with real-time fluorescence detection Ideal for daily routine diagnostics Simple and fast result analysis integrated...
结果表明:建 立的 SYBRGreenⅠ 实时荧光定量 PCR 的线性关系良好,标准曲线的相关系数达到 -1 ;最低检测限为 1.0×10 3 copies / μ L ,比常规 PCR 方法高 100 倍,与山羊痘和口蹄疫疫苗毒均不发生交叉反应;组内变异系 数为 1.061%～2.873% ,组间变异系数为 0.397%～3.829% 。用该方法检测 5 例 OrfV...