Real-life 3D therapy failure: analysis of NS5A 93H RAS plus 108K polymorphism in complex with ombitasvir by molecular modeling.computational analysisdockingHCV phosphoproteinmolecular dynamics simulationsresist
Researchers use it to measure gene expression and copy num- ber, calculate viral titers, and carry out single-nucleotide polymorphism analysis, to mention just a few applications. In particular, real-time reverse tran- scription (RT)-PCR has become the method of choice to rapidly and quanti-...
Real-time PCR combines the amplification of a DNA sequence with the detection of the amplified products during each reaction cycle—in other words, in real time. In comparison to conventional PCR, it can be used to detect a much wider range, over 107fold, of starting template concentration. ...
In real life, this data set consisted of a representative number of A. baumannii genome sequences from public databases, which were collected and processed as described above to construct the A. baumannii WGS reference data set. This A. baumannii WGS reference data set and the index isolates ...
Real-time PCR, also known as qPCR, is used for many qualitative and quantitative applications, including gene expression analysis, microRNA analysis for identification of cancer biomarkers, single nucleotide polymorphism (SNP) genotyping, copy number variation (CNV) analysis, and even protei...
Early manifestations of conventional autoimmune diseases are often subtle, much like disease-associated autoantibodies and diffuse symptoms can precede the diagnosis of, for example, rheumatoid arthritis (Rantapaa-Dahlqvist et al., 2003), anti-phospholipid syndrome (Graf, 2017; Man and Sanna, 2022) ...
Abstraction:Hiding internal details and showing functionality is known as abstraction. Consider a real-life example of a man driving a car. The man only knows that pressing the accelerators will increase the speed of car or applying brakes will stop the car but he does not know about how on...
Sanduzzi Zamparelli et al., Life, doi:10.3390/life15010113 Immune-Boosting and Antiviral Effects of Antioxidants in COVID-19 Pneumonia: A Therapeutic Perspective Review of immune-boosting and antiviral effects of antioxidants in COVID-19 pneumonia. Authors provide an overview of the literature on ...
oriented programming basic concepts e.g.Abstraction,Polymorphism,InheritanceandSOLID design principle, it’s also worth knowing useful principle like this, which has found it’s way via experience. In following example, we will see how a method can violate above rules to violate Law of...
studying DNA sequence variations, such as single nucleotide polymorphism. The basic difference from end-point PCR technique is that in addition to the qualitative result – detecting the presence of a PCR product, a quantitative result – the initial amount of the amplified product – can be ...