To demonstrate the benefit of the PROFIT-seq protocol, we simulated different DNA, cDNA and PROFIT-seq sequencing runs for 1 h using the playback function of the MinKNOW software (Methods). As shown in Fig.1b, the rejected reads in both runs had a median length of ~600 bp, with ...
In order to use the QuantiTect Multiplex PCR NoROX Master Mix on the ABI 7500 Real-time PCR System, 2 µL ROX Reference Dye (25 µM) (Invitrogen by Life Technologies, Carlsbad, CA, USA) were added to 1.8 mL master mix. As a result, the final ROX concentration was 14 ...
In brief, my argument is: (1) life is organized in a hierarchy of nested monophyletic groups-some of them quite fine-scale, well below the level we currently call species; (2) not all known monophyletic groups need be named, just the ones that are important to process or conservation ...
In daily life, Chinese people often consume mushroom ‘hotpots’, containing a variety of mushrooms. Therefore, in practical applications, the samples are usually mixed and processed. Furthermore, when mushrooms are eaten by mistake and are digested in the stomach, DNA gets degraded. Our study ...
(EBV), enter a latent stage before they start to reproduce. During latency, the virus expresses its own genes, which help maintain the viral genome until replication begins. In many viral life cycles, including EBV, reproduction continues until the cell bursts and releases a new crop of ...
WL-YOLO Wild Life—You Only Look Once CBAM Convolutional Block Attention Module YOLOv5s You Only Look Once v5—small SPP Spatial Pyramid Pooling SE Squeeze and Extraction RCNN Regional convolutional neural network FLOPS Floating-point operations CNN Convolutional neural network CUDA Computer unified dev...