Real-time RT-PCR (NDWD) adapted from Nadin-Davis et al. and Wakeley et al. [8,25] was performed using the QuantiTect Probe RT-PCR Kit (QIAGEN, Germantown, USA). The 25 μL reaction volume consisted of 9.5 μL RNase-free water, or 9 μL in the multiplex mix, 12.5 ...
Real-time PCR (quantitative PCR or qPCR) has become the preferred method for validating results obtained from assays which measure gene expression profiles. The process uses reverse transcription polymerase chain reaction (RT-PCR), coupled with fluorescent chemistry, to measure variations in transcriptome...
Comparison of Luminex xTAG RVP fast assay and real time RT‐PCR for the detection of respiratory viruses in adults with community‐acquired pneumonia 来自 国家科技图书文献中心 喜欢 0 阅读量: 20 作者: V Luchsinger,Y Prades,M Ruiz,R Pizarro,P Rossi,L Lizama,ML Garmendia,A Meza,C Larraaga,...
The aim of the study was to evaluate a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for routine diagnosis of classical swine fever (CSF) in wild boar by using 1000 spleen homogenates that were tested previously negative by virus isolation and 26 homogenates from which...
The following chemicals and reagents were obtained from commercial sources: tissues culture reagents from Gibco‐Invitrogen Corporation (Carlsbad, CA); Dex from Sigma Aldrich (St. Louis, MO); RT‐PCR reagents and Taqman‐7700 PCR instrument from PE Applied Biosystems (Branchburg, NJ); all the rea...
(C) Expression levels of TSHβ, Cga and Luc in the pituitary of TSHβLuc mice by using quantitative RT-PCR (qPCR). The statistical differences between wild-type and heterozygous or homozygous TSHβLuc mice were calculated. *P < 0.05; **P < 0.01 (two-sample t-test, n = 6–8, ...
Real‐time picosecond clocking of the collision complex in a bimolecular reaction: The birth of OH from H+CO2 BACKGROUND: The aim of this study was to use real-time polymerase chain reaction (RT-PCR) on blood samples to diagnose and serotype pneumococcal infection ... NF Scherer,LR Khundkar...
Simultaneous detection of enteric viruses by multiplex real-time RT-PCR A multiplex real-time RT-PCR protocol for the simultaneous detection of noroviruses ("Norwalk-like viruses") of genogroups I and II, human astroviruses and... C Beuret - 《Journal of Virological Methods》 被引量: 193...