Quantitative Real Time PCR (qRT‐PCR) Evaluation of Pig Mitochondrial DNA Damage 喜欢 0 阅读量: 18 作者:JL Barr,TW Thomas,RS Gibson,MA Dubick,PD Bowman 摘要: Mitochondrial DNA (MtDNA) damage has generated much interest in recent years as a potential biomarker after ischemia/reperfusion and ...
Using real‐time quantitative TaqMan RT‐PCR to evaluate the role of dexamethasone in gene regulation of rat P‐glycoproteins mdr1a/1b and cytochrome P450 3A1/2 Author links open overlay panelQin Mei, Karen Richards, Kristie Strong‐Basalyga, Scott E. Fauty, Anne Taylor, Masayo Yamazaki, ...
Aims: To optimize ethidium monoazide (EMA) coupled with real-time quantitative PCR (qPCR) and to evaluate its environmental applicability on quantifying viable legionellae in water and biofilm of cooling towers and hot water systems.Methods and Results: EMA (0·9–45·5 μg ml−1) and propi...
The real-time PCR method was used as well for the detection of other viral eye pathogens.Methods: A total of 212 patients (136 men and 76 women) suspect of having herpes simplex virus (HSV) keratitis or keratouveitis were included in the study. The detection and quantitative analysis of ...
Improved fireblight diagnostics using quantitative real‐time PCR detection of Erwinia amylovora chromosomal DNA Specific and sensitive TaqMan real-time PCR assays were developed targeting chromosomal DNA of Erwinia amylovora ( ams C gene and ITS region). These assays... M Pirc,M Ravnikar,J Toml...
is not an error source in quantitative real-time PCR assays because in such experiments the signal of the exponential phase is used for which the dye-to-base-pair ratio is larger than 2. [19] Thus, the PCR products are saturated with bound dye, and ...
Objective: The purpose of this investigation was to validate a real-time quantitative polymerase chain reaction (PCR) assay in identifying and quantifying Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis and Tannerella forsythensis from subgingival plaque samples taken from subjects with differe...
A real-time polymerase chain reaction (PCR) method developed in this study was compared with a conventional International Organization for Standardization ... TMG Catarame,KA O'Hanlon,DA Mcdowell,... - 《Journal of Food Safety》 被引量: 61发表: 2010年 Application of Quantitative Real‐Time Pol...
Novel technique of quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA. The diagnosis of tuberculous meningitis (TBM) remains a complex issue because the most widely used conventional diagnostic tools, such as culture and PCR a... Teruyuki,Takahashi,Tomohiro,... - 《Journal ...