and rapidly displaced from chromosomes in prophase, as previously reported49(Fig.4A). Indistinguishable results were obtained in HeLa cells that lacked detectable H3T3ph due to CRISPR-Cas9-mediated disruption of the Haspin gene (Fig.4A). To quantify these results, and to ensure they were...
Flow cytometry Stained cells were analyzed using an LSRII system (BD Biosciences). Data were analyzed with the Diva software v.8 (BD Biosciences) and FlowJo v.10. Cell viability was evaluated using SYTOX Blue (Life Technologies). The following antibodies were used: anti-CD5 (BD Biosciences,...
The generation of reactive oxygen species detected by flow cytometry was greatly increased in cells treated with the combination therapy, and the loss in cell viability could be effectively rescued with the reactive oxygen species inhibitor N-acetylcysteine. Moreover, enhancement of cell membrane ...
Then, the mitotic cells were released and harvested at the indicated times and subjected to flow cytometry analysis (B); the percentage of mitotic cells was calculated (C). D, cell lysates from the above experiment were subjected to immunoblotting with the indicated antibodies. Rio2 Controls ...
Results Translation efficiency of mRNAs marked by G-quadruplexes within their 5′-UTRs To identify transcripts regulated by rG4s in their 5′-UTR, we conducted transcriptome-wide ribosomal profiling [35,36] of cycloheximide-treated (i.e., translation elongation inhibited) HeLa cells. Ribosome-prof...
Cell cycle and apoptosis of Hep-2 cells were detected by flow cytometry. RESULTS: The mRNA and protein expression of 14-3-3epsilon in larynx squamous cell carcinoma (LSCC) tissues were significantly lower than those in clear surgical margin tissues. Statistical analysis showed that the 14-3-3...
Currently, its research groups address areas of neurobiology, epigenetics, developmental biology and developmental genetics and are supported by state-of-the-art core facilities in histology, recombineering/ gene editing, flow cytometry, microscopy, and mouse transgenesis. EMBL Monterotondo benefits from ...
Flow cytometryThe prion protein gene PRNP directs the synthesis of one of the most intensively studied mammalian proteins, the prion protein (PrP). Yet the physiological function of PrP has remained elusive and has created controversies in the literature. We found a downstream alternative ...
that had been sublethally irradiated (300 rads) the day before. The dilution of CellTrace Violet was assessed by flow cytometry 4 d post-transfer.f,h, Representative flow plots of CellTrace Violet dilution and the expression of CD5.g,i, Bar graphs summarizing the percentages of adoptive...
Lainer et al., “Human Lymphocyte Subpopulations Identified by Using Three-Color Immunofluorescence and Flow Cytometry Analysis: Correlation of Leu-2, Leu-3, Leu-7, Leu-8, and Leu-11 Clee Surface Antigen Expression,” Journal of Immunology, 132(1):151-156 (1984). ;132(1):151-156 (1984...