ATime-course of i3Neurons infected with ZIKV (MOI 10) for 96 h. TCID50were performed with serial dilutions of the supernatant to measure virion release. Values show the mean averages of the titration from five biological replicates. Error bars represent standard errors. Statistical analysis was...
we utilized CRISPR–Cas9 technology to introduce a mutation into the endogenous mouseLatlocus that would be transcribed as the Gly135Asp alteration (Extended Data Fig.1a–d). In LATG135Dmice, the kinetics of a single proofreading signaling step (that ...