For example, in the COSMIC dataset for the HEY2 gene, two variants are annotated as yielding a premature stop codon within the canoni- cal ORF (Q9UBP5). These two variants chr6:125,759,167 C > T and chr6:125,759,503 C > T are the only two vari- ants predicted as '...
a, A strategy for ORF mutagenesis strategy in which the start codon and downstream methionines were mutated to alanine. The shown amino acid sequence is a fictional sequence. b, A pie chart showing the number and percentage of amino acids changed per ORF from the mutagenesis. c, A violin ...
The Ribo-seq profiles of human, chimpanzee and gorilla iPSC-CMs revealed a lowTNNI3:TNNI1ratio and a highMYH7:MYH6ratio, suggesting a predominantly fetal ventricular identity29(Extended Data Fig.2d). Rhesus iPSC-CMs differed slightly, having a unique inverted ratio ofMYH7:MYH6, which, in hu...
The first exon of a protein-coding gene has to start with the start codon ATG. Thus, for the first exon, alternative candidates must start with ATG instead of sharing a theoretical splice site pattern with the first exon. The last exon is followed by a stop codon (TAG, TAA, or TGA) ...
Protein distribution (solid line graph) and density (bar graph) are shown in the bottom chart. We next performed immunocytochemistry using two cell lines that express endogenous TARP. In both LNCaP (Fig. 4A) and MDA-PCa-2b (Fig. 4B) cells labeled with TP1, we observed a dot-like pattern...
Terminal exons (or better alternative exons encoding for the terminal stop codon) might either be spliced as differentially included exons, like in the case of the Drosophila muscle myosin heavy chain gene [19], or as multiple poly(A) sites. Nevertheless, these terminal exons might contain an ...