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Therefore, genes and metabolic potentials of eukaryotic community were explored using the assembled contigs from the Hybrid assembly because of its largest size, relatively low contig number, and long contig length. By EukRep predection [86] and Kaiju annotation [87], 33 800 contigs, ca. 163 ...
Metagenome contigs were translated into proteins using FragGeneScan v1.3161, annotated taxonomically using Kaiju v1.6.062 against the proGenomes database (2017-05-16), 16S rRNA genes were identified with barrnap v0.9 (https://github.com/tseemann/barrnap), and then classified with MOTHUR v2.7.14...