Hepatitis B (HepB) vaccine was used as the stimulus; this is a monovalent vaccine consisting of HepB surface antigen (HBsAg) and alum adjuvant. We hypothesized that the HepB vaccine response might be simpler to interpret than the response to a multivalent vaccine. In the United Kingdom, ...
Most of the stem bnAbs we examined bound autoantigens; several showed staining of HEp-2 cells. A smaller proportion of the head-directed bnAbs were polyreactive. Gene usage did not correlate with autoreactivity. We suggest that complex foreign antigens may often have surface patches resembling ...
Yet, our knowledge regarding the cellular mechanisms underlying the production of tumor-reactive autoantibodies and their cell surface antigens remains elusive. Intratumoral antibody-secreting cells (ASCs) and their B cell precursors were described in many types of solid tumors, and the presence of B...
(H) Representative results for confocal analysis of human epithelial (HEp-2) cells stained with (from left to right): no primary, 564 Ab derived from a hybridoma (clone C11), cloned heavy and light chain pairs from control anti-influenza HA head Ab (6649), reconstructed 564 unmutated common...
In vitro studies have shown that IL-22BP significantly inhibits IL-22-induced activation of the STAT3 pathway in Huh-7 and HepG2 cells, and IL-22-induced activation of STAT3 is completely blocked when the IL-22BP/IL-22 ratio reaches 10 or 50 or higher [62]. In the absence of IL-...
Glycoconjugate vaccines that combine a glycan antigen with a carrier protein have for the most part replaced polysaccharide vaccines that fail to induce a strong immune response2 and do not result in B-cell memory in infants under the age of two3,4. Successful and broadly marketed carbohydrate ...
Serum samples were diluted 1:10 in PBS and placed onto HEp-2 cell-coated slides (Antibodies Incorporated) and incubated for 1 h in a humidified container. Each slide was washed with PBS before addition of FITC-conjugated anti-mouse IgG (eBiosciences) and humid incubation for an additional 1...
Analysis of one hundred antibodies recombinantly expressed from the variable region genes of single B cells demonstrated that they are predominantly autoreactive, binding to HEp-2 cell antigens and DNA. Upon BCR cross-linkage, Bcells have a reduced capacity to mobilize intracellular calcium or ...
The same applies to the proteins identified in the present screen and not known before as DNA-binding proteins (3-deoxy-d-arabinoheptulosonate-7-phosphate synthase and tryptophanase). Affinity of the former protein for AP sites could, in principle, arise from a certain resemblance of its ...
IgGg protein levels (a) and mRNA levels (b) were detected. (c) The viability of similarly treated cells was analyzed with cell proliferation assay. Results are presented as percentage of cells proliferation in comparison to the negative control. HEp-2 and PC3 cells were also treated with the...