New enzyme catalysts are usually engineered by repurposing the active sites of natural proteins. Here we show that design and directed evolution can be used to transform a non-natural, functionally naive zinc-binding protein into a highly active catalyst
Our work examines the impact of country-dyadic military conflicts on market reaction to cross-border acquisitions (CBAs). Building on intergroup relations
Liyong Zhang, Jianjun He, Jun Su, Yinji Chen, Xinyue Li, Hao Zhang, Xinzhi Jiang, Luohuan Wang, Ziming Li, Luyang Song, Yaode Sheng & Jianping Cheng Nuclear Science Laboratory, University of Notre Dame, Notre Dame, IN, USA Richard J. deBoer & Michael Wiescher School of Physics and ...
the simplified soil microcosm collected at the beginning of the experiment just after inoculation of the 11 soil microorganisms (SSM0). From 46 to 80 million paired-end reads of 100 nucleotides were obtained for each replicate and more than 84 % of them passed the quality control test (filtere...
Traditional medicine plays a crucial role as the population has little access to modern medical treatments. Voacanga africana (Scott-Elliot) Stapf is a widely distributed tree in West Africa, Congo, and Tanzania. An infusion of the root bark of VOC is traditionally used to treat a wide range...
Quantitative real-time PCR (RT-qPCR) was carried out on ten times-diluted cDNA with Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, Life Technologies) and specific primers (Additional file2), using a LightCycler 480 instrument (Roche, Branford, CT, USA). Reactions were set with two initia...
Unlocking the function promiscuity of old yellow enzyme to catalyze asymmetric Morita-Baylis-Hillman reaction Lei Wang Yaoyun Wu Wei Song Nature Communications (2024) Automated in vivo enzyme engineering accelerates biocatalyst optimization Enrico Orsi Lennart Schada von Borzyskowski Steffen N. Lindner...
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Related links Key reference using this protocol Song, P. et al. Nano Lett. 17, 5193–5198 (2017): https://doi.org/10.1021/acs.nanolett.7b01006 Key data use...
The bifunctional mechanism that involves adsorbed hydroxide in the alkaline hydrogen oxidation and evolution reactions, important in hydrogen fuel cells and water electrolysers, is hotly debated. Hydroxide binding has been suggested to impact activity, b
were used at a resonance frequency of 72.394 kHz with a tip voltage of +3.0 V with respect to the ground. The illumination during scanning was achieved by utilizing a Fiber-Liter MI-150R illuminator with a remote controller (Dolan-Jenner Industries, Boxborough, MA, USA). ...