Download: Download full-size image Fig. 5. Absorbance spectra of purified WT RC-LH1 (a), Minimal RC-LH1 (b), Chimeric RC-LH1 (c) and Tepidum LH1 (d) complexes in the ion-free state (blue), or reconstituted with 5 mM BaCl2 (grey) or CaCl2 (yellow). These properties are similar...
All other sequences were amplified using 18 μL 1.1X ReddyMix PCR Master Mix (Thermo Fisher Scientific Inc, ABGene, UK), 0.4 μL of each primer (20 μM), 0.6 μL 0.4% of bovine serum albumin (BSA, MBI-Fermentas, St. Leon-Rot, Germany), and 1–2 μL template DNA. For ...
Download: Download full-size image Figure 2. Structural features of ICab3. A, overall structure of ICab3. CDR1, CDR2, and CDR3 regions are represented by red, green, and cyan, respectively; transparent spheres depict the disulfide bond between Cys26 and Cys103. B, anomalous map (magenta ...