Ltd. The vaccine contains SARS-CoV-2 tandem-repeat dimeric form of RBD antigen (S protein 319-537, GISAID accession No. EPI_ISL_402119) manufactured in the CHO cell line, with aluminium hydroxide as the adjuvant. The adjuvant Sepivac SWE™ is a squalene-based oil-in-water adjuvant, ...
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The coverslips were then mounted on glass slides and the confocal images were obtained using a Leica TCS SP5 confocal microscope with a 63× oil immersion lens. Crystallization, Diffraction Data Collection, and Structure Determination Crystallization was performed using the hanging drop vapor diffusion ...
As expected, immunization with the RBD-NP alone did not induce robust immune response and required adjuvants to confer significant protection. Our observations are consistent with results of a human clinical trial that evaluated a self-assembling, two-component RBD-NP with non-adjuvanted or oil-in...
20 μL purified Omicron BA.2 Spike protein at the concentration of 2.3 mg/ml was incubated with 5 μL BA7535-Fab at the concentration of 3.2 mg/mL at a 1:3 molar ratio on ice for 20 min. The complex was applied for Cryo-EM grid preparation. Quantifoil Au R2/1 gri...
In total, 2 μL of S-6P (1.2 mg/mL) and 2 μL of 35B5 Fab or 32C7 Fab (0.6 mg/mL) were incubated for 3 min at room temperature and then loaded onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3). The grids were washed using the buffer cont...
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