S3, the mRNA of a single RBD-specific memory B cell was collected, and the antibody variable region cDNA was obtained by RT-PCR amplification. Specifically, when the antibody variable region cDNA was subjected to the RT-PCR amplification, primers designed in this example efficiently facilitate am...
Once all of the cables have been connected it is safe to turn on the system normally. It is recommended that the Lab6 filament and 11-065 ion gun control emission current be turned up very slowly the first time that they are used after bakeout in order to give them time to outgas....
Please note that the examples from the paper (see Citing) can be found in this repo https://github.com/s2mLab/BioptimPaperExamples. Run examples An GUI to access the examples can be run to facilitate the testing of bioptim You can either run the file __main__.py in the examples ...
MTPs were incubated overnight at 37°C and 800 rpm in a MTP shaker (Thermoshaker PST-60HL-4, Lab4You, Berlin, Germany). A volume of 180 μL 2xYT-GA in a PP-MTP well was inoculated with 20 μL of the overnight culture and grown at 37°C and 800 rpm for 90 minutes (approx....
Female New Zealand white rabbits weighing approximately 4 kg were sourced and housed at Labcorp, Inc. (Denver, PA, USA) and immunizations were performed under permits with approval number COVQ34665P. All immunization procedures complied with all relevant ethical regulations and protocols of the La...
and individuals for their assistances in the preparation of the manuscript: Professors Chuan Qin and Jiangning Liu from Institute of Laboratory Animal Sciences, CAMS & PUMC, China for their support in initial cell-based assay on neutralizing activity of the antibody generated in our lab; Dr. Lian...
(Thermoshaker PST-60HL-4, Lab4You, Berlin, Germany). A volume of 180 μL 2xYT-GA in a PP-MTP well was inoculated with 20 μL of the overnight culture and grown at 37 °C and 800 rpm for 90 min (approx. OD600of 0.5). Bacteria were harvested by centrifugation for ...
(90 mg/L of REF) using the Expi293 system; however, most studies reported values of around 20 mg/L of the RBD protein, in line with the very good yields obtained in our laboratory: 15 mg/L of S1, 12 mg/L of N, and 40 mg/L of RBD. Our lab performed transfection experiments ...
Mazalovska, M.; Varadinov, N.; Koynarski, T.; Minkov, I.; Teoharov, P.; Lomonossoff, G.P.; Zahmanova, G. Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana Benthamiana.Ann. Lab. Med.2017,37, 313. [Google Scholar...
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