3-Methyladenine (3-MA) (Selleck, Houston, TX, USA) was used to inhibit autophagy. ELISA kits for mouse interleukin (IL)-6, IL-10, and tumour necrosis factor-α (TNF-α) measurements were obtained from Jiangsu Jingmei Biotechnology Co., Ltd. (Jiangsu, China). A total RNA extraction kit...
Replied by Maegan (Houston, Texas) 03/16/2012 ★★★☆ I'm about a week or so into the acidophilus treatment, along with a few other things I'm doing. Sadie was so bad that I was about to give up. She was one hundred percent bald and completely miserable. So far I have noticed...
out by placing 500 μL of the subculture in 200 mL of MRS broth at 30 °C for 18 h, to achieve a concentration of each strain of approximately 9 log CFU/mL, adjusted by measuring absorbance at 600 nm using a spectrophotometer (Peak Instruments Inc., Version 1701, Houston, TX, USA)....
Six-well culture plates and four-well culture slides were purchased from SPL (Houston, TX, USA). Auranofin, LPS, PA, bovine serum albumin (BSA), DAPI, and CLI-095 were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Dimethyl sulfoxide (DMSO) was purchased from Amresco...