Protein samples were separated by SDS-PAGE, transferred to a polyvinylidene difluoride (PVDF) membrane, blocked with 5% skim milk in Tris-buffered saline (TBS), and probed using various antibodies. The Western blots were visualized using ECL (Bio-rad) and exposed to Amersham™ Imager 600 (GE...
without adding water. Try filling a VITA MIXER with about 1/2 green grapes, then add 1-2 apples, then celery and spinach. Blend, then either use metal strainer or nut milk bag. I use these five gallon paint strainer bags from HOME DEPOT. You pour pulp in and then squeeze with your ...