Total RNA was extracted from a single venom gland using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The integrity of total RNA was confirmed using agarose gel electrophoresis. The mRNA was purified using an mRNA isolation kit (Roche Applied Science, Mannheim, Germany). The purified total ...
Although further studies should be carried out to unveil the mechanism of action of myotoxin-3, this peptide represents a new MTA candidate isolated from an animal venom. 4. Materials and Methods 4.1. Reagents Kitoxan® and venoms, obtained from animals in captivity, were kindly donated by ...
Figure 4. ELISA-based assay for CdtVEGF recognition by anticrotalid antivenom and prediction of the recognized epitopes. (A) Samples (CdtVEGF, fraction 19, and C. d. terrificus venom—2 μg each) were incubated with commercial anticrotalid antivenom from Instituto Butantan. Data are presented ...
Samples were digested using the Calbiochem ProteoExtract All-in-one Trypsin Digestion kit (Merck, Darmstadt, Germany) with LC/MS grade solvents according to the manufacturer's instructions. The LC-MS/MS analyses were performed using an LTQ Orbitrap Velos equipped with a Nanospray Flex ion source ...
We estimated the concentration of total RNA using the Qubit RNA BR Assay (Thermo Fisher Scientific) and evaluated the quality and final concentration using an Agilent Bioanalyzer 2100 with an RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA) following the manufacturer’s instructions....