Genome editing using CRISPR-Cas systems is a promising avenue for the treatment of genetic diseases. However, cellular and humoral immunogenicity of genome editing tools, which originate from bacteria, complicates their clinical use. Here we report reduc
According to theory47, coarse-grained simulations48 and experiments49, this decreases the minimal concentration needed for phase separation. In agreement with this hypothesis, eGFP-AR-ΔNLS lacking the 23FQNLF27 motif (ΔFQNLF) formed fewer and smaller condensates in PC3 cells (Extended Data Fig....
The basic idea behind RI theory is that DMs face an abundant amount of information and cannot process all of it. However, they are aware of this limitation and decide how to process the available information optimally, trading off costs and benefits of being better informed. This idea was sug...
Genome editing using CRISPR-Cas systems is a promising avenue for the treatment of genetic diseases. However, cellular and humoral immunogenicity of genome editing tools, which originate from bacteria, complicates their clinical use. Here we report reduced immunogenicity (Red)(i)-variants of two cli...
Prediction of the lowest energy structure for transition metal clusters and related systems is fundamental in cluster science. Advances in the structure search methods have reduced the computer time for structure prediction, standing on the performance of density functional theory (DFT) calculations. This...
Although enzymes in theory offer a solution to this problem, the inherently high specificity of most enzymes involved in natural product biosynthesis restricts their use in generating modified molecules. To circumvent this limitation, enzymes may be evolved or rationally engineered to create a variant ...
The general principles of discrete, large self-assemblies composed of numerous components are not unveiled and the artificial formation of such entities is a challenging topic. In metal–organic cages, design strategies for tuning the coordination direct
The general principles of discrete, large self-assemblies composed of numerous components are not unveiled and the artificial formation of such entities is a challenging topic. In metal–organic cages, design strategies for tuning the coordination direct
When fitting to this data, we used the standard error of the pKD extracted from the Langmuir isotherm fit to each construct/pH condition as the standard error for each corresponding data point. We fit pH-dependence curves with the functional form: $$p{\it{K}}_{\rm{D}} = \Delta p{\...
We consider a variational two-dimensional Landau–de Gennes model in the theory of nematic liquid crystals in a disk of radius R. We prove that under