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A minimum of 500 consensus sequences per experiment was required to determine the error rate. To avoid counting mutations present in the DNA used for RNA transcription, mutations present in more than 95% of the consensus sequences were discarded. Statistical analysis was conducted using the Tukey...
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Differences in biochemical reaction rates, including the rates of protein production and degradation, are thought to be responsible for species-specific rates of development1,2,3. However, the cause of differential biochemical reaction rates between species remains unknown. Here, using pluripotent stem ...
For each gene, an infinite number of combinations of transcription and translation rates can achieve a given protein abundance. For example, to obtain 1000 proteins per hour, one possibility is to translate 1000 proteins per hour from a single mRNA. Another option is to translate 10 proteins per...
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This is a critical detail for a rigorous analysis of agreement which is necessary to be able to replicate a study, since otherwise it is not clear under what conditions a human transcription should count as having agreed with an automatic classification or not. Xu et al. further reported a ...
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selections utilizing cell-based technologies such as fluorescence-activated cell sorting (FACS), continuous culturing devices (Wong et al., 2018), and droplet screening systems; and other selections that have been developed for rewiring the specificities of biosensors, GPCRs, or transcription factors ...
(2), especially in the par- ticular problem of gene-regulation by highly specific transcription-factors. Previously, Stéfanini, McKane and Newman14 solved a model with input of single substrate-molecules into a small cellular compart- ment which acts as the reaction volume. Their formula for ...