Co-culture of rat luteal cells with islet cells enhances islet viability and revascularizationFunctionalityProgesteroneType I diabetesVascular endothelial growth factorVascularizationIslet cell transplantation is a major treatment strategy for type I diabetes, and has proven to be effective for maintaining ...
Long-term culture and coculture of primary rat and human hepatocytes. Methods Mol Biol. 2013;945:287-302.Long-term culture and coculture of primary rat and human hepatocytes. Shulman M,Nahmias Y. Epithelial Cell Culture Protocols . 2013
We have produced a murine monoclonal antibody (F43 A1D2) that binds to the cell surface of both rat islet tumor cells (RINm clone 5F and RINm clone 14B) and normal rat islet cells. This antibody is cytotoxic in the presence of complement... MA Crump,R Scearce,M Dobersen,... - 《...
centrifugation could separate the cell successfully.High purification but low cell yield was obtained with immunomagnetic beads.The cells handled with Thy1.1 antibody had both higher purify coefficient and higher yield.Conclusion The developed method could separate the brain microendothelial cells ...
brain endothelial celltight junctionPEGylatednanoparticletransport of nanoparticlePoly(MePEG 2000 cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles have demonstrated their capacity to reach the rat central nervous system after intravenous injection. For insight into the transport of colloidal ...
Cluture Conditions of the Primary Rat Aortic Endothelial Cells (AEC) DMEM/F12+5% FBS+1% Endothelial Cell Growth Supplement+1% Penicillin-Streptomycin Solution. Temperature: 37℃. Condition: 95% air, 5% carbon dioxide Product Format of the Primary Rat Aortic Endothelial Cells (AEC) Frozen 1 mL ...
Cell cycle analysis was done after co-culturing for 1 day both in the mono-culture control group and in co-culture groups. Percentages of cells at each stage of the cell cycle were analysed (A). [(G2 + M/S)/G1] ×100% of cells in each group was calculated to evaluate the...
We have developed and characterized a new syngeneic BBB model using primary cultures of the three main cell types of cerebral microvessels. The co-culture of endothelial cells, pericytes and astrocytes mimick the anatomical situation in vivo. In the presence of both pericytes and astrocytes rat ...
Objectives To establish a culture approach of smooth muscle cells and analyze cell growth and biological characteristics. Methods The thoracic aorta of rat was separated carefully and cut into small tissue pieces. These explants were seeded onto culture flasks and cultured in complete medium consisting...
A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells Article Open access 08 January 2025 Hepatocyte organoids and cell transplantation: What the future holds Article Open access 18 October 2021 Introduction The liver is the largest organ in the human body, ...