RAT DISSECTION Wikispaces大鼠解剖Wikispaces.ppt,FEMALE RAT DISSECTION NIGEL CARDOZO 11B BIOLOGY CIRCULATORY SYSTEM The blood returns from the lower body through the inferior vena cava and the blood returns through the upper body from the right and left
The dissection was performed from dorsum to plantar sequentially. The first dorsal metatarsal artery was cauterized, the extensor hallucis longus divided, and then the first metatarsophalangeal joint was disarticulated. The first metatarsal bone was removed to expose the muscular layers under this ...
Distraction osteogenesis is accompanied by significant neovascularization, and research indicates that bone distraction elevates the expression of pro-angiogenic factors like VEGF and SDF-1 in the circulatory system. This increase coincides with an uptick in the number of endothelial progenitor cells, ...
After dissection, fibers were placed in ice-cold BIOPS-buffer supplemented with 50 μg·mL−1 Saponin for 30 minutes to ensure permeabilization. Fibers were subsequently washed by agitation in ice-cold MiR05-buffer (composition in mM): EGTA 0.5, MgCl2·6H2O 3.0, K-lactobionate 60, ...
Animal dissection Directly after declaring termination of vital functions, the chest cavity was opened. The inferior vena cava and descending aorta were cannulated, all blood was removed, and infusion of the body using large volumes of Ringer’s solution (Fresenius Kabi, Germany) was conducted in...
pipette grinder air stone and dissection microscope--see reference in text or purchase a package from Sutter Instruments or World Precision Instruments RX Honing Machine, System II RX Honing Machine Corporation MAC-10700 Rx System II Machine alternative for air stone, use with a dissecting microscope...
Single-cell dissection of the human brain vasculature Article14 February 2022 Introduction As in all vascular beds, brain mural cells are classically defined as the only cells to be entirely embedded in the vascular basement membrane1. They are mesenchymal cells that, in the brain, are mostly der...
Approximately equal sized hemispheres from each rat brain were used for gene microarray analysis (see above for extraction and dissection method). RNA was isolated from frozen brain tissue using TRIZOL (Life Technologies, Carlsbad, CA) and stored at −80 °C. Total RNA quality was assessed ...
Cardiomyocytes were dissociated from ventricles dis- sected from Sprague-Dawley rat pups at postnatal day 3 and maintained in cold dissection buffer (in mM: NaCl 1at3270, K0 Cg lfo5r.356 m, Ming,StOhe4 c0e.8ll1s,wdeerxetrroesseus5p.5e5n,dKeHd 2inPOth4e0c.4u4lt,uNrae2mHePdOiu...
a respiratory mask was placed on the rat’s face with 60 breaths per min and 7 mL per breath after anesthesia. The left chest and intercostal muscles between the third and fourth ribs were separated via blunt dissection. Ribs were then cut off after ligation, and the heart was exposed ...