We describe here a new related gene named , isolated from a cDNA library of immortalized simian B-lymphocytes. The gene codes for a 206 amino acid protein of expected mol. wt 23.5 kd that shares greater than 50% homology with H-, K-or N-. The regions of and a C-terminal cysteine ...
The RhoA pRAICHU probe uses human RhoA as the sensor region and Rho-binding domain of the protein kinase PKN as the ligand region. Upon GTP binding, the effector region of RhoA binds to PKN, which triggers FRET between CFP and YFP. B and C, measurement of FRET efficiency by confocal ...