We have separated multiple small Mr GTP-binding proteins (G proteins) from bovine brain membranes by several column chromatographies and purified to near homogeneity four of them, including a novel Mr 24,000 G protein (smg p25A), a novel Mr 22,000 G protein (smg p21), the rho protein ...
A novel regulatory protein for the rho proteins (rhoA p21 and rhoB p20), belonging to a ras p21/ras p21-like small molecular weight (Mr) GTP-binding protein (G protein) superfamily, was purified to near homogeneity from bovine brain cytosol and characterized. This regulatory protein, ...
During this process, the hydrolysis of GTP acts as an energy source as well as an on-off switch for the GTPase activity of the LMW GTP-binding proteins. The LMW GTP-binding proteins are classified into five subfamilies: Ras, Rho, Ran, Rab, and ADP-ribosylation factor. Despite their ...
Ras guanine nucleotide-releasing protein (RasGRP) is one type of GEF (GTP/GDP exchange factor) responsible for the activation of Ras and/or Rap14,15. Each protein contains a Ras exchange motif to interact with Ras and/or Rap, CDC25, EF hands for Ca2+ binding and C1 domain for diacylgly...
Ras, the archetype of low molecular weight GTP-binding proteins, first was identified in mammalian cells through gain-of-function mutations in human tumors, corresponding to the transforming genes of Harvey and Kirsten murine sarcoma viruses (1, 2, 3). Ras subsequently was reported to function, ...
The surface of the Cdc73 C-domain that is equivalent to the canonical GTP-binding pocket is relatively flat. Using this surface to interact with another protein would bypass the need for a deep binding pocket. Any of the proteins involved in the transcription complex could be potential binding...
1999. Sec14p-like domains in NF1 and Dbl-like proteins indicate lipid regulation of Ras and Rho signaling. Curr. Biol. 9:195.Aravind L, Neuwald AF, Ponting CP. Sec14p-like domains in NF1 and Dbl-like proteins indicate lipid regulation of Ras and Rho signaling. Curr Biol 1999;9:R...
Aravind L, Neuwald AF, Ponting CP (1999) Sec14p-like domains in NF1 and Dbl-like proteins indicate lipid regulation of Ras and Rho signaling [letter]. Curr Biol 9(6):R195–R197Aravind L, Neuwald AF, Ponting CP. Sec14p-like domains in NF1 and Dbl-like proteins indicate lipid ...