Ras-ERK信号通路细胞增殖凋亡目的探讨辛伐他汀作用K562细胞后的Ras-细胞外信号调节激酶(ERK)信号转导通路分子水平的变化,以说明辛伐他汀通过引起Ras-ERK信号通路分子水平的变化参与K562细胞细胞增殖和细胞凋亡的调节.方法体外用辛伐他汀处理慢性粒细胞自血病细胞株K562细胞,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖抑制...
异常的表观调控下调miR-663促进原癌基因H.ras在K562细胞系 的表达 中文摘要 microRNA(miRNA)是一类19到24个核苷酸大小的非编码小RNA,在转录后 水平和翻译水平,通过与靶基因mRNA的3’非编码区(uTR)结合,在转录后和 翻译水平抑制靶基因的表达.已有的研究表明,抑癌基因启动子区的异常甲基化、 ...
姜黄素对K562细胞增殖的影响与P210“。m61 激活的Ras信号途径和细胞周期蛋白的关系 硕士研究生:吴丽贤 导师:**华教授 摘要 姜科姜黄属植物姜黄(Curcuma longa L。)是一种常用中药,味辛、苦, 性温,无毒。姜黄素(Curcumin,Cur)是从姜黄中提取的酚性色素一悔 ...
摘要 AIM: To investigate the effects of curcumin (Cur) on proliferation of K562 cells and the relationship between these effects and Ras signal transduction pathway activated by p210bcr/abl METHODS: K562 cell line was used as a p210bcr/abl-positive cell system and HL-60 cell line as a p210...
人原发性肝癌及7402细胞株和髓细胞白血病细胞株(K562)的癌基因(转化基因)的共同属性——N-ras基因 用人的原发性肝癌(PHC),肝癌7402细胞株(郭婵等,1984)及髓细胞白血病K562细胞株的DNA转染的NIH/3T3转化株DNA,以Southern吸印和~(32)P-标记的癌基因探针做分子杂交来分... 顾健人,田培坤,王翔,... - 《中国...
The ras mutant, pZIPRas61leu-transfected cells, K562-Ras61leu, have shown a markedly decreased cell proliferation rate with approximately 2-fold doubling time, compared with the parental K562 cells, and about 60% of these cells have shown the phenotype of erythroid differentiation. In addition,...
To investigate the effects of curcumin (Cur) on proliferation of K562 cells and the relationship between these effects and Ras signal transduction pathway activated by p210. K562 cell line was used as a p210-positive cell system and HL-60 cell line as a p210-negative control; etoposide (VP-...
A mutant human N-ras gene (codon 61, C to A substitution) was electroporated into the human leukemic cell line K562, originally derived from a patient with chronic myeloid leukemia (CML) in blast crisis. Despite confirmation of mutant N-ras gene integration and expression, mutant transfected ...
(1994) Induction of apoptosis by a dominant negative H-RAS mutant (116Y) in K562 cells. Exp. Cell Res., 215, 131-136.Sakai N, Ogiso Y, Fujita H, Watari H, Koike T and Kuzumaki N (1994) Induction of apoptosis by a dominant negative H-RAS mutant (116Y) in K652 cells. Exp. ...
p21RasandProliferatingCellNuclearAntigenGenein ErythroleukemiaCellLineK562 WANGYa—li(王雅莉),LUOYa-ii(骆亚莉),CHENChe(陈 彻),LINeng—lian(李能莲) SHEYa-li(舍雅莉),andZHANGLi(张 立) ABsTRACT Objective:ToinvestigatetheefectoftotalflavonoidsofHedysarumpolybotryontheproliferation. cellcycle,andexpressions...