First, each DNA molecule is prepared with a short insert size and then sequenced with overlapping paired-end (PE) reads. The reads are then merged into a single, high-quality consensus sequence that is free of sequencing errors. Second, during library generation each sample is PCR amplified ...
It is worth noting, though, that in all of the mouse RTT models examined to date, the lack of proper MeCP2 function has promoted the hyper-excitability of neural networks in the brain. It will be interesting to determine if the mechanisms underlying the network hyper-excitability in these ...