A high concentration of thrombin quickly and specifically cleaved the introduced site between the tags and the target fragment. We found that thrombin tightly bound to an ion-exchange resin, CM-Sepharose, under conditions avoiding adsorption of most proteins. By passing through the column, thrombin ...
Hoving S, Munchbach M, Schmid H, et al. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification[J]. Anal Chem, 2000, 72: 1006-1014.Hoving S, Mtmchbach M, Schmid H. A method for chemical generation of N-terminal peptide tags for rapid...