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(Waltham, MA, USA). PBS tablets were purchased from Abcam BioVision (Waltham, MA, USA). Sucrose came from PanReac AppliChem (Barcelona, Spain). All aqueous solutions and buffers were prepared using pure water obtained from a Milli-Q water system (Merck, Darmstadt, Germany), with a ...
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Harvested cell pellets were thawed on ice and resuspended in lysis buffer containing 1 mM Pefabloc (Roth) and cOmplete Ultra tablets, EDTA free (Merck) protease inhibitor to minimize protein degradation. Sonification steps were performed on ice for efficient cell lysis (6 10-s bursts with cooling...
As aforementioned, the method produces fine dry particles containing protein and a coprecipitant that can be redissolved rapidly in aqueous solution and are thus also attractive for the production of cleansing agents that contain enzymes. Enzymes can be incorporated into tablets, creams, powders, gels...