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Reverse transcriptasepolymerase chain reaction(RT-PCR) and rapid polymerase chain reaction (PCR) are two different techniques used to amplify genetic material in a cell. The main difference between RT-PCR and rapid PCR is the type of molecule that is being amplified.RT-PCR amplifies the RNA, wh...
We demonstrate that the relative effectiveness of RA tests and RT-PCR testing in reducing post-quarantine transmission depends on the quarantine duration and the turnaround time of testing results. For quarantines of two days or shorter, conducting a RA test on exit from quarantine reduces onward ...
The drug susceptibility testing of M. leprae by the RT-PCR based assay was found to be not only faster but also nearly -fold more sensitive than the Buddmeyer-type assay. Moreover, it was also found that, unlike the RT-PCR based assay, the same testing by a DNA-PCR resulted in no ...
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Real-time PCR has been used previously to detect Yersinia pestis; this study applies this rapid, specific, and sensitive nucleic acid-based method to the detection and quantitation of Y. pestis specifically in food. Five sets of primers and corresponding TaqMan dual-labelled fluorogenic hybridization...
本发明公开一种鉴别掺假肉及其制品的多重PCR快速检测方法. The present invention discloses a multiplex PCR assay for rapid detection method to identify adulteration of meat and its products. 传统的检测方法检测时间长,耗时,耗力. Traditional testing methods for a long time, time-consuming, labor-intensiv...
This PCR analysis was successfully employed to identify F. moniliforme directly from the mycelia that develop from naturally infected maize seeds, with no need to obtain pure fungal cultures for reliable diagnosis. The protocol can be used for the diagnosis of infected plants and soils in ...
These results show that testing by PCR for the presence of the 278-bp sequence is a rapid and reliable method for sex identification. 展开 关键词: forest musk deer sex identification ZFX/ZFY special primers multiplex PCR DOI: 10.2108/zsj.24.493 ...
Of the new cases, 30,877 were done through traditional PCR testing and a further 61,387 were self-reported by citizens since the government opened the self-reporting system on Wednesday. The surge in cases has been largely attributed to the Omicron variant, which has quickly outpaced PCR test...