Rapidity versus riability: Critical analysis of polymerase chain reaction (PCR) as a new rapid diagnostic technique in mycobacterial infectionsTintelnot, KKirchner, PSchmidt, MBottger, E CHeesemann, J
The sensitivity of culture versus PCR was 83.3% (95% CI: 67.2–93.6) for culture performed on site and 72.2% (95% CI: 54.8–85.8) at the international reference laboratory, where samples were tested after an average delay of two months after sample collection, and specificity was 98.5% (...
The TAT, i.e., time from sampling to a reported test result, varied considerably by method (Table2). Median TAT for the FAPpluswas strikingly shorter, compared with in-house PCR (2.6 vs 24.1 h, median difference of 21.0 h (IQR 16.3–24.7), p < 0.001) and sputum cultures (both...
For quarantine durations varying from one to 14 days, we compared the probability of PQT when performing a single RT-PCR test on exit to a RA test on exit or RA tests on both entry and exit. The objective of the additional RA test on entry to the one on exit is to compensate for t...
This review provides moderate support for the strategy of usingLFAsas a ‘triage’ test forEVDin symptomatic patients. LFAs are both sensitive and specific for EVD diagnosis in the context of patients presenting to the ETUs with symptoms consistent with EVD, pending final PCR testing. In field ...
versus 99·4%, with whole blood[34]. This test can detect all HCV genotypes and gives the results in 20 min: a pink colour change that can be read at naked eye. It is a reliable and easy-to-use test, perfect for diagnosis at point-of-care[35]. However, OraQuick-HCV test price...
Xpert MTB/RIF versus sputum microscopy as the initial diagnostic test for tuberculosis: a cluster-randomised trial embedded in south African roll-out of Xpert MTB/RIF. Lancet Glob Heal. 2015;3:e450–7. Article Google Scholar Lawn SD, Kerkhoff AD, Vogt M, Wood R. Clinical significance of ...
The objective was to compare a PCR-based test performed in the laboratory versus labor ward. 300 patients were included prospectively. In phase I, swabs were analyzed by selective culture and rapid PCR in the laboratory. In phase II, swabs were analyzed accordingly, but the PCR test was ...
Student's t test was used to compare creatine phosphate (PCr)/adenosine triphosphate (ATP) ratios from the cardiac patients with those of the control subjects (n = 13). RESULTS: The myocardial PCr/ATP ratio obtained by rapid 31P-MRS was significantly lower (P < 0.001) in DCM patients (...
PCR. For this first RT-PCR test, the US Centres for Disease Control and Prevention N2-gene assay17as well as the Alberta Precision Labs E-gene assay16were performed. For the E-gene assay, 5 µL of extracted RNA was added to 2.5 µL of TaqMan Fast Virus One-Step RT-PCR Master ...