2*Rapid Tap master MIX(绿色) 10ul Primer 各0.1ul ddH2O Xul(用来补充体积至20ul) DNA 1ul 温控: Vazyme Biotech -2 × Rapid Taq Master Mix说明书 *引物对一般有两种,野生型特异性(W)和突变型特异性(M) *一定要设置阳性(突变个体),阴性(野生型个体)和空白对照组(水) *当我们需要去区分的基因型...
To tap into this unexplored diversity and expand the repertoire of Cas9s available for genome targeting applications, the development of a method that allows the direct read-out of Cas9 endonuclease PAM specificity as a function of Cas9-guide RNA complex concentration in vitro is reported. Briefly...
Mice had ad libitum access to standard chow and tap water until the induction of hyponatremia according to previously described methods with some modification [46]. Briefly, osmotic minipumps (Alzet® model 1004; Durect Corporation, Cupertino, CA) containing desmopressin (dDAVP) (10 mg/mL; ...
with light intensities reaching 2000 µmol photons m−2 s−1at mid-day24,25. They show stronger induction of NPQ relative to algae living in other habitats; furthermore, in laboratory settings mimicking fluctuating light, estuarine and coastal diatoms show greater resistance...
20) whereas this cessation has not occurred at 60 min in the TAP medium (Supplementary Fig. 19). We further caution that the difference between FL and CL in C. reinhardtii is in part due to the large fold increase in the CL samples. To confirm the functional relevance of the above ...
There may be some areas you find that can be streamlined, but don't rush through it and mix up the very similar looking small drill bits or break more taps! The basic process is: Use the sprocket hub plate as a template to drill, tap, install 1 screw, to rotationally lock it in ...
RT-PCR was performed on RNA isolated from sour orange leaves that had been syringe-infiltrated with either the Xcc pathogen or autoclaved tap water. Overall, the group of seedlings with high ROS production (SO53, SO56, and SO57) showed an increase in the level of transcript of the PTI-re...
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Human metapnemovirus F gene NC_004148 469 Human coronavirus 229E/NL63 S gene 229E: NC_002645 375 NL63: NC_005831 Human parainfluenzavirus 1 HN gene NC_003461 324 Human parainfluenzavirus 2 HN gene NC_003443 264 Human parainfluenzavirus 3 HN gene NC_001796 219 Respiratory virus 1-B set...
PCR amplification was performed in a total volume of 20 µL, containing 5 µL DNA, 10 µL of AccuPower 2X GreenStar qPCR Master Mix (Bioneer, Daejeon, Korea), 2.5 µM of each primer, and 25 µM HSSP on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Waltham, MA,...