5´–CAU CAU CAU CAU GAC CGT TCA GCT GGA TAT TAC–3´ In each of three fresh 0.5-ml microcentrifuge tubes, prepare a PCR mixture as in step 1 of Protocol 2, substituting 1 μl of the control GSP for the user-defined GS...
A set of compatible tac promoter expression vectors. Gene. 1996. https://doi.org/10.1016/0378-1119(96)00289-2. Article PubMed Google Scholar Oyston PCF, Sjostedt A, Titball RW. Tularaemia: Bioterrorism defence renews interest in Francisella tularensis. Nat Rev Microbiol. 2004. https://doi....
a step that traditionally requires SDS-PAGE. The measurement immobilizes the heavy and light chains of the sdFab to the AlphaLISA beads, resulting in signal when the two chains are assembled (Supplementary Fig.5a). The AlphaLISA assembly assay generally...
The time-activity curves (TAC) of blood, brain, and liver radioactivities at 0, 4, and 21 h were analyzed with Prism 8 (Graphpad). Blood TACs were fitted with one phase decay. Radioactivity density from image analysis is presented as percent injected dose per cubic centimeter (% ID/cc...
J. Oncogenic driver FGFR3-TACC3 is dependent on membrane trafficking and ERK signaling. Oncotarget. 9(76), 34306–34319 (2018). Article PubMed PubMed Central Google Scholar Gallo, L. H., Nelson, K. N., Meyer, A. N. & Donoghue, D. J. Functions of fibroblast growth factor receptors...
Bromodomain and extra-terminal domain (BET) proteins are therapeutic targets in several cancers including the most common malignant adult brain tumor glioblastoma (GBM). Multiple small molecule inhibitors of BET proteins have been utilized in preclinical
Clonotype-specific probes used were 3A11div (5′-ACC CTC AGG GGC GGT GTA-3′), 6G2div (5′-TTG TGG TCG GGG GGG TCC TA-3′), 3B12div (5′-AGC CAA ACA GAG GGG CTG TAC-3′), and 12.5B10div (5′-CCG TGG ACA GGG GGC TCG TAT-3′). Filters were then washed twice in 6 ...
18. Foerstner KUK, von Mering CC, Hooper SDS, Bork PP: Environments shape the nucleotide composition of genomes. EMBO Rep 2005, 6(12):1208–1213. doi:10.1038/sj.embor.7400538. 19. Karlin S, Mrazek J: Compositional differences within and between eukaryotic genomes. Proc Natl Acad Sci U ...
(Supplementary Fig.15b–e). We further observed that the M3Kδ6-FLAG protein band in SDS-PAGE gels was slightly shifted in response to 15 min osmotic stress treatment in mesophyll cell protoplasts, suggesting an osmotic stress-dependent post-translational modification of M3Kδ6 (Fig.6f;n...
(Supplementary Fig.15b–e). We further observed that the M3Kδ6-FLAG protein band in SDS-PAGE gels was slightly shifted in response to 15 min osmotic stress treatment in mesophyll cell protoplasts, suggesting an osmotic stress-dependent post-translational modification of M3Kδ6 (Fig.6f;n...