To test these tools, we profiled samples from preterm infants who were clinically diagnosed with NEC26(Supplementary Fig.2a,b). Samples from infants P49 and P205 both contained high proportions ofE. cloacae(Fig.3a,b), with the correlation plots of normalized reads assigned at 1 and 6 h ...
PfAgo protein was purified using the established protocol11. Briefly, a strep(II)-tagged (N-terminal) codon-optimized version ofPfAgo gene was cloned into pET28a plasmid. The expression plasmid was transformed intoEscherichia coliKRX (Promega) followed by induction and purification. The purified prot...
AlphaLISA detection was performed using Anti-Mouse IgG Alpha Donor beads (PerkinElmer, AS104) and Strep-Tactin AlphaLISA Acceptor beads (PerkinElmer, AL136). IC50 values were calculated using Prism 9 by fitting the normalized data to [Inhibitor] vs. response–Variable slope (four parameters) fit ...
Clinical samples are contaminated more than desired with low levels of ubiquitous bacteria such as Streptoccocus or Staphyloccocus. Elimination of unintended contamination during sample processing is of utmost importance in clinical diagnosis. Addition of a non-target specific sequence at a large copy...
(Pen/Strep; Gibco), 1% GlutaMAX (Gibco), and 1× MEM with nonessential amino acids (Sigma-Aldrich). Upon confluence, microglia were “shaken off” manually and plated in dishes coated with poly-l-lysine (PLL; Peptides International, 0.01%) and Collagen IV (Corning, 4 μg/ml) in ...
Each spring, male deer sprout a new pair of antlers, which are essentially temporary external bones, at a speed unparalleled by the bone growth of other mammals. Now, research led by scientists at the Stanford University School of Medicine has identified
its own. Given that NanoControl binds RBC, but lacks a viral antigen, this protein controls efficiently for non-specific (antigen-independent) cross-linking activity. We note that the αStrep AB is a monoclonal IgG1 AB, suggesting that the valency of IgG is sufficient to trigger agglutination...
AlphaLISA detection was performed using Anti-Mouse IgG Alpha Donor beads (PerkinElmer, AS104) and Strep-Tactin AlphaLISA Acceptor beads (PerkinElmer, AL136). IC50 values were calculated using Prism 9 by fitting the nor- malized data to [Inhibitor] vs. response–Variable slope (four para- ...
Taking into account its strong stability, ClyR has great potential to be an effective therapeutic agent against strep- tococcal mediated infections. This study also provides, for the first time, an induced lysis-based rapid, visual screening method for finding active and soluble peptidoglycan ...
the results from throat culture, molecular respiratory pathogen panels, and the following blood tests: CBC including WBC and a diff with bands and lymphocytes, procalcitonin, Epstein-Barr virus IgM/IgM as well as additional standard of care tests performed including rapid flu and rapid strep tests...