Approximately 400 ng total DNA from adult OR and ID beetles was used for the RADseq protocol. We developed a customized single restriction enzyme digest protocol similar to Etter and colleagues77, with additional DNA cleaning steps and a slightly modified PE2 adapter (available upon request). ...
Midnight protocol For Oxford Nanopore sequencing, the Midnight primer kit was used as described previously54. cDNA synthesis was performed on the extracted RNA using the LunaScript RT mastermix (New England BioLabs) followed by gene-specific multiplex PCR using the Midnight primer pools, which produc...
21 In this context, we hypothesize that the variation in T cell proliferative response and the lack of its correlation with type 1 IFN responses may be explained by differential levels of T cell priming in individual participants. We also identified a similarly rapid B cell response represented ...
To address this question, we performed whole-cell patch-clamp recordings in acute slices in mice that were exposed to 3 h of EE as outlined in Fig.4a. The passive properties were unchanged between the two groups (RMP: Ctrl –86.59 ± 5.53 mV, EE –88.56 ± 5.70 mV...
This trend can be explained by the splice junctions found in human transcriptome sequences. With fewer bases, the odds of a read spanning a splice junction are smaller and the read will be more likely to align. Conversely, when aligning reads against the bacterial and viral libraries, the ...
Input resistance (RN) was calculated from the slope of the current/voltage relationship curve from a current clamp step protocol. Resting membrane potential (RMP) was measured directly upon entering whole-cell configuration in current clamp at I = 0. AP properties were measured with a step ...